生物微粒的分離和操控在生物晶片上的應用

Separation and manipulation of bio-particles applied in bio-chip

指導教授 : 黃榮堂、 陳正光    研究生 : 張圍勝  機電整合研究所 92年


摘要

利用介電泳力(Dielectrophoresis Force) 的特性來分離或操控生物微粒的技術已經存在一段時間了,但是都只能單獨的分離或操控生物微粒。本研究為了將其整合至整合型生物晶片上,所以必須同時做到分離與操控生物微粒,並將經過分離後之生物微粒控制到整合型晶片的下一站。

本研究主要先探討介電泳力的數學模型,比較傳統介電泳力(cDEP)和移動式介電泳力(twDEP)在不同生物微粒介電力的差異。再參考此數值來進行分離和操控的實驗,也利用模擬軟體(CFD-RC)模擬出電場的強度與方向,並可利用它來模擬生物微粒在一介質溶液中受到介電力的移動情況,比對理論和模擬的狀況再和實際實驗相互驗證。

在晶片的實際製作上,本研究利用微機電製程技術在玻璃基材上製作出微電極,並採用上下雙層電極的方式,讓晶片形成3D的結構,這種做法主要可以改善單層電極無法完全控制流道內分佈不均之粒子。並利用ISA (Industry Standard Architecture) 標準匯流排插槽將輸出訊號輸出到電極上,可以提高實驗的穩定性和方便性,並可重複的使用。

最後本研究成功的將淋巴細胞與酵母菌分離開來,並且使淋巴細胞沿著流道移動,同時達到分離與操控的目的。

關鍵詞:分離,生物晶片,介電力

ABSTRACT

Employing the property of DEP force to separate or manipulate bio-particles has already existed over two decades, but it just can be used to separate or manipulate bio-particles solely. In this research, in order to integrate the technology onto the integrated bio-chip, we must separate and manipulate these bio-particles simultaneously, and then precisely control the separated bio-particles to the next station of integrated biochip.

In this thesis, we primarily discuss the mathematical model of DEP force as well as compare the DEP differences under different bio-particles by using cDEP and twDEP. After that, we further refer these data to do the experiments of separation and manipulation. Furthermore, we also use CFD-RC, a simulation software, to simulate the strength and distribution of electro-field. And then utilize the results to simulate the bio-particles’ movement condition influenced by DEP force in a medium solution and compare the different results among theory, simulation and experiment. We make use of MEMS process to fabricate the micro-electrode on the glass substrate during chip fabrication procedure. We adopt double-layers micro-electrode, which can make the chip form 3D structure in this design. This method is able to improve the problem of particles non-uniform distribution in the micro-channel causing by single layer micro-electrode design. In addition, by using ISA Bus slot and connecting output signal on the electrode, we can enhance the stability, convenience, and receptivity of this experiment. Finally, we successfully separate and manipulate the lymphocyte from the yeast, and control lymphocyte to travel along the micro-channel precisely, which means we can achieve the goal of separation and manipulation simultaneously.

Keywords:Separation, Manipulation, Bio-chip, Dielectrophoresis